Antsa Raherimanana
Master 2 student
The 3D-Hub-O organoid production platform specializes in the production, manipulation and analysis of organoids from normal and pathological tissues. 3D-Hub-O puts its expertise to work for the academic and industrial communities. It is committed to improving human health through its contribution to personalized medicine.
Skills and expertise
- 2 proposed organoid culture techniques:
- Or immersed drop culture
- Or filter culture at the air-liquid interface
Activities and services
- Organoid production from normal and pathological tissues
- Setting up biobanks
- Organoid manipulation by electroporation or lentivirus infection
- Organoid analysis (morphological studies, viability tests)
- Drug testing
- Inclusion
- Co-cultures
- Advice and training in organoid production and analysis
Equipment
- Leica M205FA stereomicroscope in Noroit Class II fume hood: Motorized contrast and fluorescence acquisition (GFP/Ds Red) under sterile conditions
- 2 Hera cell vios incubators: 6 cell lockers/incubator
- Sonidel electroporator : Nepa 21 for in vitro transfection
- Evos M7000 microscope : automated imaging, monochrome and color CMOS camera, colorimetry, 4 colors (GFP , Ds Red, mCherry, Dapi)
- EVOS M 5000 inverted microscope : 5-channel color imaging (transmission, GFP , Ds Red, mCherry, Dapi)
- COUNTESS II FL automated cell counter : 3 channels (transmission, GFP , Ds Red)
- Temperature-controlled Orbital incubator shaker
- Zebra ZT231 barcode label printer : 300dpi thermal transfer
Featured Publications
06/2024
B. Sarri, V. Chevrier, F. Poizat, S. Heuke, F. Franchi, L. De Franqueville, E. Traversari, J.P . Ratone, F. Caillol, Y. Dahel, S. Hoibian, M. Giovannini, C. de Chaisemartin, R. Appay, G. Guasch and H. Rigneault.
06/2023
E. Traversari, J. Bonnet, M. Saubin, S. Triaire, F. Poizat, J.P . Ratone, Y. Dahel, F. Caillol, C. Pesanti, O. Comiti, M. Giovannini, C. De Chaisemartin, O. Turrini, C. Ginestier, E. Charafe-Jauffret, G. Guasch and V. Chevrier.
06/2022
L. Richart, M.-L. Picod, M. Wassef, M. Macario, S. Aflaki, M.A. Salvador, J. Wicinski, V. Chevrier, S. Le Cam, H.A. Kamhawi, R. Castellano, G. Guasch, E. Charafe-Jauffret, E. Heard, R. Margueron and C. Ginestier.
05/2021
L. Mitoyan, V. Chevrier, H. Hernandez-Vargas, A. Ollivier, Z. Homayed, J. Pannequin, F. Poizat, C. Biasi-Cador, E. Charafe-Jauffret, C. Ginestier and G. Guasch.
Labels, Funding and Partners
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Like others, they were part of the team. Thank you to all those who have contributed to CRCM's excellence and impact.
