Nous sommes ravis d’annoncer la publication de notre dernier article dans Cell : « Recognition of BACH1 quaternary structure degrons by two F-box proteins under oxidative stress ». Ce travail, mené en collaboration avec plusieurs équipes de recherche, explore comment deux E3 ligases distinctes reconnaissent et ciblent les degrons quaternaires de BACH1 en réponse au stress oxydatif. Une avancée clé pour mieux comprendre la régulation de la protéostasie et la réponse cellulaire au stress !

Abstract : Ubiquitin-dependent proteolysis regulates diverse cellular functions with high substrate specificity, which hinges on the ability of ubiquitin E3 ligases to decode the targets’ degradation signals, i.e., degrons. Here, we show that BACH1, a transcription repressor of antioxidant response genes, features two distinct unconventional degrons encrypted in the quaternary structure of its homodimeric BTB domain. These two degrons are both functionalized by oxidative stress and are deciphered by two complementary E3s. FBXO22 recognizes a degron constructed by the BACH1 BTB domain dimer interface, which is unmasked from transcriptional co-repressors after oxidative stress releases BACH1 from chromatin. When this degron is impaired by oxidation, a second BACH1 degron manifested by its destabilized BTB dimer is probed by a pair of FBXL17 proteins that remodels the substrate into E3-bound monomers for ubiquitination. Our findings highlight the multidimensionality of protein degradation signals and the functional complementarity of different ubiquitin ligases targeting the same substrate.

En Savoir Plus : Cao S, Garcia SF, Shi H, James EI, Kito Y, Shi H, Mao H, Kaisari S, Rona G, Deng S, Goldberg HV, Ponce J, Ueberheide B, Lignitto L, Guttman M, Pagano M, Zheng N. Recognition of BACH1 quaternary structure degrons by two F-box proteins under oxidative stress. Cell. 2024 Dec 26;187(26):7568-7584.e22. doi: 10.1016/j.cell.2024.10.012. Epub 2024 Nov 5. PMID: 39504958; PMCID: PMC11682927.